Mouse TrkB ELISA Kit PicoKine®

TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that in humans is encoded by the NTRK2 gene.[1] TrkB is the high affinity catalytic receptor for several neurotrophins, which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The TrkB receptor is part of the large family of receptor tyrosine kinases. A tyrosine kinase is an enzyme which is capable of adding a phosphate group to certain tyrosines on target proteins, or substrates.Soppet et al. [2] demonstrated that the gp145 gene product of the TRKB gene is rapidly phosphorylated on tyrosine residues upon exposure to BDNF and NTF3. The standard product used in this kit is recombinant TrkB, a 49.5KDa glycoprotein, C32—H439.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 187 195 201 192 5.67 2.9% Sample 2 679 781 662 676 699 47.48 6.7% Sample 3 2008 2037 2196 2162 2100 79.82 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 187 195 201 192 5.67 2.9% Sample 1 188 187 195 201 192 5.67 2.9% Sample 2 679 781 662 676 699 47.48 6.7% Sample 2 679 781 662 676 699 47.48 6.7% Sample 3 2008 2037 2196 2162 2100 79.82 3.8% Sample 3 2008 2037 2196 2162 2100 79.82 3.8%

BDNF/NT-3 growth factors receptor;2.7.10.1; GP145-TrkB/GP95-TrkB; Trk-B; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Ntrk2; Trkb

Widely expressed in the central and peripheral nervous system. The different forms are differentially expressed in various cell types. Isoform GP95-TRKB is specifically expressed in glial cells.

Cancer, Growth and Development, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Oncoproteins, Oncoproteins/Suppressors, Pathways and Processes

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 679 2008 202 622 2201 Standard deviation 12.22 46.85 128.51 13.73 46.02 149.66 CV (%) 6.5% 6.9% 6.4% 6.8% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 679 2008 202 622 2201 Standard deviation 12.22 46.85 128.51 13.73 46.02 149.66 CV (%) 6.5% 6.9% 6.4% 6.8% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 188 679 2008 202 622 2201 Mean (pg/ml) 188 679 2008 202 622 2201 Standard deviation 12.22 46.85 128.51 13.73 46.02 149.66 Standard deviation 12.22 46.85 128.51 13.73 46.02 149.66 CV (%) 6.5% 6.9% 6.4% 6.8% 7.4% 6.8% CV (%) 6.5% 6.9% 6.4% 6.8% 7.4% 6.8%

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin- 4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane ; Single-pass type I membrane protein . Endosome membrane ; Single-pass type I membrane protein . Internalized to endosomes upon ligand-binding.

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.032 0.097 0.154 0.235 0.409 0.722 1.329 2.243 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.032 0.097 0.154 0.235 0.409 0.722 1.329 2.243 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.032 0.097 0.154 0.235 0.409 0.722 1.329 2.243 O.D. 0.032 0.097 0.154 0.235 0.409 0.722 1.329 2.243

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.