Mouse TNFSF11/RANKL ELISA Kit PicoKine®

Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 39 32 37 35 2.69 7.6% Sample 2 195 191 201 186 193 5.49 2.8% Sample 3 430 475 480 482 466 21.37 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 39 32 37 35 2.69 7.6% Sample 1 34 39 32 37 35 2.69 7.6% Sample 2 195 191 201 186 193 5.49 2.8% Sample 2 195 191 201 186 193 5.49 2.8% Sample 3 430 475 480 482 466 21.37 4.5% Sample 3 430 475 480 482 466 21.37 4.5%

Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor; ODF; Osteoprotegerin ligand; OPGL; Receptor activator of nuclear factor kappa-B ligand; RANKL; TNF-related activation-induced cytokine; TRANCE; CD254; Tumor necrosis factor ligand superfamily member 11, membrane form; Tumor necrosis factor ligand superfamily member 11, soluble form; Tnfsf11; Opgl, Rankl, Trance

Highly expressed in thymus and lymph nodes, but not in non-lymphoid tissues and is abundantly expressed in T- cells but not in B-cells. A high level expression is also seen in the trabecular bone and lung.

Adaptive Immunity, Atherosclerosis, Cardiovascular, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Immunology, Innate Immunity, Signal Transduction, Structures, TNF Superfamily

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 132 430 32 199 419 Standard deviation 1.42 10.72 17.63 1.72 12.13 21.36 CV (%) 4.2% 5.5% 4.1% 5.4% 6.1% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 132 430 32 199 419 Standard deviation 1.42 10.72 17.63 1.72 12.13 21.36 CV (%) 4.2% 5.5% 4.1% 5.4% 6.1% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 132 430 32 199 419 Mean (pg/ml) 34 132 430 32 199 419 Standard deviation 1.42 10.72 17.63 1.72 12.13 21.36 Standard deviation 1.42 10.72 17.63 1.72 12.13 21.36 CV (%) 4.2% 5.5% 4.1% 5.4% 6.1% 5.1% CV (%) 4.2% 5.5% 4.1% 5.4% 6.1% 5.1%

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.094 0.162 0.314 0.561 0.973 1.544 2.063 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.094 0.162 0.314 0.561 0.973 1.544 2.063 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.006 0.094 0.162 0.314 0.561 0.973 1.544 2.063 O.D. 0.006 0.094 0.162 0.314 0.561 0.973 1.544 2.063

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.