Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine®

Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine® (96 Tests) . Quantitate Human TFPI in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TFPI (Tissue factor pathway inhibitor), also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa) . The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 57 66 64 61 4.06 6.6% Sample 2 238 272 240 245 248 13.66 5.5% Sample 3 848 982 998 845 918 71.98 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 57 66 64 61 4.06 6.6% Sample 1 57 57 66 64 61 4.06 6.6% Sample 2 238 272 240 245 248 13.66 5.5% Sample 2 238 272 240 245 248 13.66 5.5% Sample 3 848 982 998 845 918 71.98 7.8% Sample 3 848 982 998 845 918 71.98 7.8%

Synonyms

Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI; TFPI; LACI, TFPI1

Gene Name

TFPI

Gene ID

7035

UniProt

P10646

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D29-K282

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Mostly in endothelial cells.

Applications

ELISA

Field of Research

Cardiovascular, Platelets

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 238 848 55 237 897 Standard deviation 3.3 11.9 66.14 3.46 14.93 75.34 CV (%) 5.8% 5% 7.8% 6.3%% 6.3% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 238 848 55 237 897 Standard deviation 3.3 11.9 66.14 3.46 14.93 75.34 CV (%) 5.8% 5% 7.8% 6.3%% 6.3% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 238 848 55 237 897 Mean (pg/ml) 57 238 848 55 237 897 Standard deviation 3.3 11.9 66.14 3.46 14.93 75.34 Standard deviation 3.3 11.9 66.14 3.46 14.93 75.34 CV (%) 5.8% 5% 7.8% 6.3%% 6.3% 8.4% CV (%) 5.8% 5% 7.8% 6.3%% 6.3% 8.4%

Sensitivity

<10 pg/mL

Function

Inhibits factor X (X (a) ) ly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

35015 MW

Specificity

Natural and recombinant human TFPI

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tissue factor pathway inhibitor

Subcellular Location

Isoform Alpha: Secreted.

Sequence Similarities

Contains 3 BPTI/Kunitz inhibitor domains.

Protein Name

Tissue factor pathway inhibitor

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.078 0.128 0.24 0.412 0.753 1.434 2.337 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.078 0.128 0.24 0.412 0.753 1.434 2.337 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.078 0.128 0.24 0.412 0.753 1.434 2.337 O.D. 0.016 0.078 0.128 0.24 0.412 0.753 1.434 2.337

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.