Human Kallistatin/Serpina4 ELISA Kit PicoKine®

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 260 258 290 262 17.34 6.6% Sample 2 1648 1727 1660 1766 1700 48.44 2.8% Sample 3 4379 4465 4565 4726 4533 129.04 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 260 258 290 262 17.34 6.6% Sample 1 242 260 258 290 262 17.34 6.6% Sample 2 1648 1727 1660 1766 1700 48.44 2.8% Sample 2 1648 1727 1660 1766 1700 48.44 2.8% Sample 3 4379 4465 4565 4726 4533 129.04 2.8% Sample 3 4379 4465 4565 4726 4533 129.04 2.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1648 4379 232 1601 4027 Standard deviation 11.37 95.58 214.57 12.76 94.45 249.67 CV (%) 4.7% 5.8% 4.9% 5.5% 5.9% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1648 4379 232 1601 4027 Standard deviation 11.37 95.58 214.57 12.76 94.45 249.67 CV (%) 4.7% 5.8% 4.9% 5.5% 5.9% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 242 1648 4379 232 1601 4027 Mean (pg/ml) 242 1648 4379 232 1601 4027 Standard deviation 11.37 95.58 214.57 12.76 94.45 249.67 Standard deviation 11.37 95.58 214.57 12.76 94.45 249.67 CV (%) 4.7% 5.8% 4.9% 5.5% 5.9% 6.2% CV (%) 4.7% 5.8% 4.9% 5.5% 5.9% 6.2%

Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.075 0.125 0.242 0.469 0.803 1.337 2.03 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.075 0.125 0.242 0.469 0.803 1.337 2.03 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.075 0.125 0.242 0.469 0.803 1.337 2.03 O.D. 0.015 0.075 0.125 0.242 0.469 0.803 1.337 2.03

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.