Human NOV/CCN3 ELISA Kit PicoKine®

Human NOV/CCN3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CCN3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

NOV (nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 61 66 62 63 2.27 3.6% Sample 2 265 294 297 292 287 12.82 4.4% Sample 3 820 835 750 802 801 32.08 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 61 66 62 63 2.27 3.6% Sample 1 66 61 66 62 63 2.27 3.6% Sample 2 265 294 297 292 287 12.82 4.4% Sample 2 265 294 297 292 287 12.82 4.4% Sample 3 820 835 750 802 801 32.08 4% Sample 3 820 835 750 802 801 32.08 4%

Synonyms

Protein NOV homolog; NovH; CCN family member 3; Insulin-like growth factor-binding protein 9; IBP-9; IGF-binding protein 9; IGFBP-9; Nephroblastoma-overexpressed gene protein homolog; NOV; CCN3, IGFBP9, NOVH

Gene Name

NOV

Gene ID

4856

UniProt

P48745

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: T32-M357

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. .

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 265 820 61 258 754 Standard deviation 2.77 16.43 37.72 3.41 19.86 44.2%4 CV (%) 4.2% 6.2% 4.6% 5.6% 7.7% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 265 820 61 258 754 Standard deviation 2.77 16.43 37.72 3.41 19.86 44.2%4 CV (%) 4.2% 6.2% 4.6% 5.6% 7.7% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 66 265 820 61 258 754 Mean (pg/ml) 66 265 820 61 258 754 Standard deviation 2.77 16.43 37.72 3.41 19.86 44.2%4 Standard deviation 2.77 16.43 37.72 3.41 19.86 44.2%4 CV (%) 4.2% 6.2% 4.6% 5.6% 7.7% 6% CV (%) 4.2% 6.2% 4.6% 5.6% 7.7% 6%

Sensitivity

<2 pg/mL

Function

Immediate-early protein likely to play a role in cell growth regulation. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

39162 MW

Specificity

Natural and recombinant human CCN3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Protein NOV homolog

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the CCN family.

Protein Name

Protein NOV homolog

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.045 0.079 0.161 0.338 0.67 1.288 2.425 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.045 0.079 0.161 0.338 0.67 1.288 2.425 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.045 0.079 0.161 0.338 0.67 1.288 2.425 O.D. 0.007 0.045 0.079 0.161 0.338 0.67 1.288 2.425

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.