Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine®

Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that it is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard product used in this kit are recombinant mouse B7-1/CD80, D37—K245, consisting of dimer acids with two single straned. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 418 421 430 389 414 15.37 3.7% Sample 2 2343 2347 2512 2667 2467 133.97 5.4% Sample 3 8435 8668 9897 9494 9123 595.2 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 418 421 430 389 414 15.37 3.7% Sample 1 418 421 430 389 414 15.37 3.7% Sample 2 2343 2347 2512 2667 2467 133.97 5.4% Sample 2 2343 2347 2512 2667 2467 133.97 5.4% Sample 3 8435 8668 9897 9494 9123 595.2 6.5% Sample 3 8435 8668 9897 9494 9123 595.2 6.5%

Atherosclerosis, Cancer, Cardiovascular, Cofactors, Vitamins/Minerals, Diabetes, Diabetes-associated, Heart Disease, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction, Vitamins/Minerals

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 418 2343 8435 401 2576 7635 Standard deviation 23.4 105.43 649.49 28.07 159.71 633.7 CV (%) 4.5% 4.5% 7.7% 7% 6.2% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 418 2343 8435 401 2576 7635 Standard deviation 23.4 105.43 649.49 28.07 159.71 633.7 CV (%) 4.5% 4.5% 7.7% 7% 6.2% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 418 2343 8435 401 2576 7635 Mean (pg/ml) 418 2343 8435 401 2576 7635 Standard deviation 23.4 105.43 649.49 28.07 159.71 633.7 Standard deviation 23.4 105.43 649.49 28.07 159.71 633.7 CV (%) 4.5% 4.5% 7.7% 7% 6.2% 8.3% CV (%) 4.5% 4.5% 7.7% 7% 6.2% 8.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.087 0.133 0.228 0.411 0.747 1.44 2.227 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.087 0.133 0.228 0.411 0.747 1.44 2.227 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.087 0.133 0.228 0.411 0.747 1.44 2.227 O.D. 0.022 0.087 0.133 0.228 0.411 0.747 1.44 2.227

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.