Mouse RAGE ELISA Kit PicoKine®

RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 113 119 116 113 115 2.48 2.1% Sample 2 797 796 771 805 792 12.75 1.6% Sample 3 2158 2179 2112 2263 2178 54.73 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 113 119 116 113 115 2.48 2.1% Sample 1 113 119 116 113 115 2.48 2.1% Sample 2 797 796 771 805 792 12.75 1.6% Sample 2 797 796 771 805 792 12.75 1.6% Sample 3 2158 2179 2112 2263 2178 54.73 2.5% Sample 3 2158 2179 2112 2263 2178 54.73 2.5%

Alzheimer's Disease, Atherosclerosis, Cardiovascular, Diabetes-associated, Neurodegenerative Disease, Neurology Process, Neuroscience, Sensory System, Vascular Inflammation, Visual System

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 113 797 2158 103 830 2180 Standard deviation 4.63 55.79 90.63 6%5 61.42 130.8 CV (%) 4.1% 7% 4.2% 5% 7.4% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 113 797 2158 103 830 2180 Standard deviation 4.63 55.79 90.63 6%5 61.42 130.8 CV (%) 4.1% 7% 4.2% 5% 7.4% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 113 797 2158 103 830 2180 Mean (pg/ml) 113 797 2158 103 830 2180 Standard deviation 4.63 55.79 90.63 6%5 61.42 130.8 Standard deviation 4.63 55.79 90.63 6%5 61.42 130.8 CV (%) 4.1% 7% 4.2% 5% 7.4% 6% CV (%) 4.1% 7% 4.2% 5% 7.4% 6%

Mediates interactions of advanced glycosylation end products (AGE) . These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Can also bind oligonucleotides. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. RAGE-dependent signaling in microglia contributes to neuroinflammation, amyloid accumulation, and impaired learning/memory in a mouse model of Alzheimer disease. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.022 0.089 0.147 0.254 0.452 0.789 1.306 1.892 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.022 0.089 0.147 0.254 0.452 0.789 1.306 1.892 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.022 0.089 0.147 0.254 0.452 0.789 1.306 1.892 O.D. 0.022 0.089 0.147 0.254 0.452 0.789 1.306 1.892

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.