Human Rage ELISA Kit PicoKine®

RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 153 141 152 150 5.24 3.4% Sample 2 623 570 627 596 604 22.96 3.8% Sample 3 3076 3035 3093 3144 3087 39.08 1.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 153 141 152 150 5.24 3.4% Sample 1 154 153 141 152 150 5.24 3.4% Sample 2 623 570 627 596 604 22.96 3.8% Sample 2 623 570 627 596 604 22.96 3.8% Sample 3 3076 3035 3093 3144 3087 39.08 1.2% Sample 3 3076 3035 3093 3144 3087 39.08 1.2%

Atherosclerosis, Cardiovascular, Diabetes-associated, Neurodegenerative Disease, Neurology Process, Neuroscience, Sensory System, Vascular Inflammation, Visual System

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 623 3076 159 595 3344 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 623 3076 159 595 3344 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 154 623 3076 159 595 3344 Mean (pg/ml) 154 623 3076 159 595 3344 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5% CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.084 0.156 0.204 0.319 0.419 0.628 1.014 1.819 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.084 0.156 0.204 0.319 0.419 0.628 1.014 1.819 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.084 0.156 0.204 0.319 0.419 0.628 1.014 1.819 O.D. 0.084 0.156 0.204 0.319 0.419 0.628 1.014 1.819

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.