Human LOX-1/OLR1 ELISA Kit PicoKine®

LOX-1 (lectin-type oxidized LDL receptor 1) also known as OLR1, is a protein that in humans is encoded by the OLR1 gene. LOX-1 is a receptor protein which belongs to the C-type lectin superfamily. The LOX1 gene is mapped to 12p13-p12 by fluorescence in situ hybridization. LOX1 is expressed on the plasma membrane of differentiated macrophages, but not on monocytes. The LOX1 protein acts as a macrophage scavenger receptor. LOX1 expression was detected in all choroidal neovascular membranes, regardless of structure, whereas there was no evidence of LOX1 within the posterior segments of normal eyes. LOX1 plays an active role in the pathogenesis of choroidal neovascularization, especially in ARMD.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 77 81 83 82 3.96 4.8% Sample 2 211 220 195 196 205 10.5 5.1% Sample 3 833 881 979 973 916 61.9 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 77 81 83 82 3.96 4.8% Sample 1 88 77 81 83 82 3.96 4.8% Sample 2 211 220 195 196 205 10.5 5.1% Sample 2 211 220 195 196 205 10.5 5.1% Sample 3 833 881 979 973 916 61.9 6.7% Sample 3 833 881 979 973 916 61.9 6.7%

Oxidized low-density lipoprotein receptor 1; Ox-LDL receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1; Oxidized low-density lipoprotein receptor 1, soluble form; OLR1; CLEC8A, LOX1

Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 211 833 90 192 784 Standard deviation 6.86 12.02 64.14 8.46 10.94 69.77 CV (%) 7.8% 5.7% 7.7% 9.4% 5.7% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 211 833 90 192 784 Standard deviation 6.86 12.02 64.14 8.46 10.94 69.77 CV (%) 7.8% 5.7% 7.7% 9.4% 5.7% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 211 833 90 192 784 Mean (pg/ml) 88 211 833 90 192 784 Standard deviation 6.86 12.02 64.14 8.46 10.94 69.77 Standard deviation 6.86 12.02 64.14 8.46 10.94 69.77 CV (%) 7.8% 5.7% 7.7% 9.4% 5.7% 8.9% CV (%) 7.8% 5.7% 7.7% 9.4% 5.7% 8.9%

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane; Lipid-anchor. Cell membrane; Single-pass type II membrane protein. Membrane raft. Secreted. A secreted form also exists. Localization to membrane rafts requires palmitoylation.

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.035 0.074 0.109 0.17 0.308 0.59 1.019 1.863 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.035 0.074 0.109 0.17 0.308 0.59 1.019 1.863 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.035 0.074 0.109 0.17 0.308 0.59 1.019 1.863 O.D. 0.035 0.074 0.109 0.17 0.308 0.59 1.019 1.863

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.