Human KLK5/Kallikrein-5 ELISA Kit PicoKine®

Human KLK5/Kallikrein 5 ELISA Kit PicoKine® (96 Tests) . Quantitate Human KLK5 in cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 128 140 148 137 7.39 5.3% Sample 2 809 798 776 808 797 13.27 1.6% Sample 3 2161 2628 2234 2197 2305 188.26 8.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 128 140 148 137 7.39 5.3% Sample 1 134 128 140 148 137 7.39 5.3% Sample 2 809 798 776 808 797 13.27 1.6% Sample 2 809 798 776 808 797 13.27 1.6% Sample 3 2161 2628 2234 2197 2305 188.26 8.1% Sample 3 2161 2628 2234 2197 2305 188.26 8.1%

Synonyms

Kallikrein-5;3.4.21.-; Kallikrein-like protein 2; KLK-L2; Stratum corneum tryptic enzyme; KLK5; SCTE; UNQ570/PRO1132

Gene Name

KLK5

Gene ID

25818

UniProt

Q9Y337

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V23-S293

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin. .

Applications

ELISA

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), human milk and saliva.

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 809 2161 146 747 2368 Standard deviation 5.36 33.97 133.98 8.32 32.12 149.18 CV (%) 4% 4.2% 6.2% 5.7% 4.3% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 809 2161 146 747 2368 Standard deviation 5.36 33.97 133.98 8.32 32.12 149.18 CV (%) 4% 4.2% 6.2% 5.7% 4.3% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 134 809 2161 146 747 2368 Mean (pg/ml) 134 809 2161 146 747 2368 Standard deviation 5.36 33.97 133.98 8.32 32.12 149.18 Standard deviation 5.36 33.97 133.98 8.32 32.12 149.18 CV (%) 4% 4.2% 6.2% 5.7% 4.3% 6.3% CV (%) 4% 4.2% 6.2% 5.7% 4.3% 6.3%

Sensitivity

<10 pg/mL

Function

May be involved in desquamation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

32019 MW

Specificity

Natural and recombinant human KLK5

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Kallikrein-5

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein Name

Kallikrein-5

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.065 0.105 0.175 0.311 0.657 1.054 1.877 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.065 0.105 0.175 0.311 0.657 1.054 1.877 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.065 0.105 0.175 0.311 0.657 1.054 1.877 O.D. 0.025 0.065 0.105 0.175 0.311 0.657 1.054 1.877

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.