Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine®

Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine® (96 Tests) . Quantitate Human MST1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Macrophage Stimulating 1 (SMT1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21) . The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 250 301 271 291 278 19.56 7% Sample 2 1105 1177 1061 1161 1126 46.07 4% Sample 3 6318 6544 5798 6359 6254 277.1 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 250 301 271 291 278 19.56 7% Sample 1 250 301 271 291 278 19.56 7% Sample 2 1105 1177 1061 1161 1126 46.07 4% Sample 2 1105 1177 1061 1161 1126 46.07 4% Sample 3 6318 6544 5798 6359 6254 277.1 4.4% Sample 3 6318 6544 5798 6359 6254 277.1 4.4%

Synonyms

Hepatocyte growth factor-like protein; Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; Hepatocyte growth factor-like protein alpha chain; Hepatocyte growth factor-like protein beta chain; MST1; D3F15S2, DNF15S2, HGFL

Gene Name

MST1

Gene ID

4485

UniProt

P26927

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-G711

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Monoclonal antibody from mouse

Applications

ELISA

Field of Research

Cancer, Immunology, Innate Immunity, Macrophage/Inflammation, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 250 1105 6318 245 1017 6055 Standard deviation 19.25 44.2 398.03 23.52 53.9 399.63 CV (%) 7.7% 4% 5.3%% 9.6% 5.3% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 250 1105 6318 245 1017 6055 Standard deviation 19.25 44.2 398.03 23.52 53.9 399.63 CV (%) 7.7% 4% 5.3%% 9.6% 5.3% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 250 1105 6318 245 1017 6055 Mean (pg/ml) 250 1105 6318 245 1017 6055 Standard deviation 19.25 44.2 398.03 23.52 53.9 399.63 Standard deviation 19.25 44.2 398.03 23.52 53.9 399.63 CV (%) 7.7% 4% 5.3%% 9.6% 5.3% 6.6% CV (%) 7.7% 4% 5.3%% 9.6% 5.3% 6.6%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

80320 MW

Specificity

Natural and recombinant human MSP

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Hepatocyte growth factor-like protein

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the peptidase S1 family. Plasminogen subfamily.

Protein Name

Hepatocyte growth factor-like protein

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.121 0.183 0.317 0.543 0.968 1.566 2.037 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.121 0.183 0.317 0.543 0.968 1.566 2.037 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.121 0.183 0.317 0.543 0.968 1.566 2.037 O.D. 0.036 0.121 0.183 0.317 0.543 0.968 1.566 2.037

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.