Human MIF ELISA Kit PicoKine®

Macrophage migration inhibitory factor (MIF) is a protein which in humans is encoded by the MIF gene.1, 2 This gene is located to human chromosome 22q11.2.3 It is remarkably small; it has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb.4 This gene encodes a lymphokine that may be involved in cell-mediated immunity, immunoregulation, and inflammation.5 MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein might form a complex in the cytosol near the peripheral plasma membrane, which may possibly indicate a role in integrin signaling pathways. MIF also plays a central role in the toxic response to endotoxemia and possibly septic shock.Macrophage migration inhibitory factor has been reported to interact with COP9 constitutive photomorphogenic homolog subunit 5, CD74, BNIPL, and CXCR4.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 280 266 256 273 268 8.87 3.3% Sample 2 1655 1562 1783 1751 1687 86.54 5.1% Sample 3 4559 4531 3899 4612 4400 290.85 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 280 266 256 273 268 8.87 3.3% Sample 1 280 266 256 273 268 8.87 3.3% Sample 2 1655 1562 1783 1751 1687 86.54 5.1% Sample 2 1655 1562 1783 1751 1687 86.54 5.1% Sample 3 4559 4531 3899 4612 4400 290.85 6.6% Sample 3 4559 4531 3899 4612 4400 290.85 6.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 280 1655 4559 305 1694 4885 Standard deviation 12.04 66.2 206.35 16.47 77.92 307.75 CV (%) 4.3% 4% 4.5% 5.4% 4.6% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 280 1655 4559 305 1694 4885 Standard deviation 12.04 66.2 206.35 16.47 77.92 307.75 CV (%) 4.3% 4% 4.5% 5.4% 4.6% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 280 1655 4559 305 1694 4885 Mean (pg/ml) 280 1655 4559 305 1694 4885 Standard deviation 12.04 66.2 206.35 16.47 77.92 307.75 Standard deviation 12.04 66.2 206.35 16.47 77.92 307.75 CV (%) 4.3% 4% 4.5% 5.4% 4.6% 6.3% CV (%) 4.3% 4% 4.5% 5.4% 4.6% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.067 0.161 0.272 0.468 0.859 1.412 2.0 2.432 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.067 0.161 0.272 0.468 0.859 1.412 2.0 2.432 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.067 0.161 0.272 0.468 0.859 1.412 2.0 2.432 O.D. 0.067 0.161 0.272 0.468 0.859 1.412 2.0 2.432

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.