Human Mer ELISA Kit PicoKine®

Proto-oncogene tyrosine-protein kinase MER is an enzyme that in humans is encoded by the MERTK gene. This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Its gene is mapped to chromosome 2q14.1. Mer encodes a 984-amino acid protein with a calculated molecular mass of 109 kD. It is expressed in numerous neoplastic B- and T-cell lines. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP)

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 266 272 241 253 15.49 6.1% Sample 2 1665 1842 1731 1814 1763 69.76 3.9% Sample 3 4937 4427 4544 5104 4753 277.04 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 266 272 241 253 15.49 6.1% Sample 1 236 266 272 241 253 15.49 6.1% Sample 2 1665 1842 1731 1814 1763 69.76 3.9% Sample 2 1665 1842 1731 1814 1763 69.76 3.9% Sample 3 4937 4427 4544 5104 4753 277.04 5.8% Sample 3 4937 4427 4544 5104 4753 277.04 5.8%

Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.

Intracellular Signaling, Kinases, Neuroscience, Neurotransmission, Protein Phosphorylation, Receptor Tyrosine Kinases, Sensory System, Signal Transduction, Tyrosine Kinases, Visual System

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1665 4937 217 1701 4920 Standard deviation 14.63 79.92 197.48 13.67 105.46 221.4 CV (%) 6.2% 4.8% 4% 6.2%% 6.2% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1665 4937 217 1701 4920 Standard deviation 14.63 79.92 197.48 13.67 105.46 221.4 CV (%) 6.2% 4.8% 4% 6.2%% 6.2% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 236 1665 4937 217 1701 4920 Mean (pg/ml) 236 1665 4937 217 1701 4920 Standard deviation 14.63 79.92 197.48 13.67 105.46 221.4 Standard deviation 14.63 79.92 197.48 13.67 105.46 221.4 CV (%) 6.2% 4.8% 4% 6.2%% 6.2% 4.5% CV (%) 6.2% 4.8% 4% 6.2%% 6.2% 4.5%

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis) . Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll- like receptors (TLRs) -mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.039 0.115 0.181 0.335 0.584 1.093 1.734 2.219 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.039 0.115 0.181 0.335 0.584 1.093 1.734 2.219 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.039 0.115 0.181 0.335 0.584 1.093 1.734 2.219 O.D. 0.039 0.115 0.181 0.335 0.584 1.093 1.734 2.219

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.