Human CCL13/MCP4 ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 19 17 16 17 1.29 7.5% Sample 2 85 88 83 82 84 2.29 2.7% Sample 3 256 236 245 254 247 7.94 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 19 17 16 17 1.29 7.5% Sample 1 19 19 17 16 17 1.29 7.5% Sample 2 85 88 83 82 84 2.29 2.7% Sample 2 85 88 83 82 84 2.29 2.7% Sample 3 256 236 245 254 247 7.94 3.2% Sample 3 256 236 245 254 247 7.94 3.2%

C-C motif chemokine 13; CK-beta-10; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; MCP-4; NCC-1; Small-inducible cytokine A13; C-C motif chemokine 13, long chain; C-C motif chemokine 13, medium chain; C-C motif chemokine 13, short chain; CCL13; MCP4, NCC1, SCYA13

Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 85 256 18 88 273 Standard deviation 1.14 7 13.05 1.26 6.3 19.11 CV (%) 6% 6% 7% 7% 6.4% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 85 256 18 88 273 Standard deviation 1.14 7 13.05 1.26 6.3 19.11 CV (%) 6% 6% 7% 7% 6.4% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 19 85 256 18 88 273 Mean (pg/ml) 19 85 256 18 88 273 Standard deviation 1.14 7 13.05 1.26 6.3 19.11 Standard deviation 1.14 7 13.05 1.26 6.3 19.11 CV (%) 6% 6% 7% 7% 6.4% 7% CV (%) 6% 6% 7% 7% 6.4% 7%

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.032 0.075 0.124 0.204 0.349 0.688 1.151 1.881 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.032 0.075 0.124 0.204 0.349 0.688 1.151 1.881 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.032 0.075 0.124 0.204 0.349 0.688 1.151 1.881 O.D. 0.032 0.075 0.124 0.204 0.349 0.688 1.151 1.881

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.