Human XCL1/Lymphotactin ELISA Kit PicoKine®
Human XCL1/Lymphotactin ELISA Kit PicoKine® (96 Tests) . Quantitate Human XCL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Product Specifications
Background
Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 129 130 134 146 134 6.75 5% Sample 2 466 420 445 456 446 17.13 3.8% Sample 3 1208 1267 1389 1234 1274 69.33 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 129 130 134 146 134 6.75 5% Sample 1 129 130 134 146 134 6.75 5% Sample 2 466 420 445 456 446 17.13 3.8% Sample 2 466 420 445 456 446 17.13 3.8% Sample 3 1208 1267 1389 1234 1274 69.33 5.4% Sample 3 1208 1267 1389 1234 1274 69.33 5.4%
Synonyms
Lymphotactin; ATAC; C motif chemokine 1; Cytokine SCM-1; Lymphotaxin; SCM-1-alpha; Small-inducible cytokine C1; XC chemokine ligand 1; XCL1; LTN, SCYC1
Gene Name
XCL1
Gene ID
6375
UniProt
P47992
Reactivity
Human
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V22-G114
Capture Antibody
Monoclonal antibody from mouse
Detection Antibody
Polyclonal antibody from goat
Tissue Specificity
Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.
Applications
ELISA
Field of Research
Chemokines, Immunology, Innate Immunity
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
Detection Range
62.5 pg/mL - 4,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 129 466 1208 118 435 1181 Standard deviation 8.51 30.75 54.36 9.2 36.6%3 72.04 CV (%) 6.6% 6.6% 4.5% 7.8% 8.1% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 129 466 1208 118 435 1181 Standard deviation 8.51 30.75 54.36 9.2 36.6%3 72.04 CV (%) 6.6% 6.6% 4.5% 7.8% 8.1% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 129 466 1208 118 435 1181 Mean (pg/ml) 129 466 1208 118 435 1181 Standard deviation 8.51 30.75 54.36 9.2 36.6%3 72.04 Standard deviation 8.51 30.75 54.36 9.2 36.6%3 72.04 CV (%) 6.6% 6.6% 4.5% 7.8% 8.1% 6.1% CV (%) 6.6% 6.6% 4.5% 7.8% 8.1% 6.1%
Sensitivity
<1 pg/mL
Function
Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular Weight
12517 MW
Specificity
Natural and recombinant human XCL1
Anticoagulant
Heparin or EDTA
Gene Name Synonym
Lymphotactin
Subcellular Location
Secreted.
Sequence Similarities
Belongs to the intercrine gamma family.
Protein Name
Lymphotactin
Sample Data
Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.112 0.16 0.302 0.514 0.977 1.686 2.343 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.112 0.16 0.302 0.514 0.977 1.686 2.343 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.112 0.16 0.302 0.514 0.977 1.686 2.343 O.D. 0.036 0.112 0.16 0.302 0.514 0.977 1.686 2.343
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
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