Human XCL1/Lymphotactin ELISA Kit PicoKine®

Human XCL1/Lymphotactin ELISA Kit PicoKine® (96 Tests) . Quantitate Human XCL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 129 130 134 146 134 6.75 5% Sample 2 466 420 445 456 446 17.13 3.8% Sample 3 1208 1267 1389 1234 1274 69.33 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 129 130 134 146 134 6.75 5% Sample 1 129 130 134 146 134 6.75 5% Sample 2 466 420 445 456 446 17.13 3.8% Sample 2 466 420 445 456 446 17.13 3.8% Sample 3 1208 1267 1389 1234 1274 69.33 5.4% Sample 3 1208 1267 1389 1234 1274 69.33 5.4%

Synonyms

Lymphotactin; ATAC; C motif chemokine 1; Cytokine SCM-1; Lymphotaxin; SCM-1-alpha; Small-inducible cytokine C1; XC chemokine ligand 1; XCL1; LTN, SCYC1

Gene Name

XCL1

Gene ID

6375

UniProt

P47992

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V22-G114

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 129 466 1208 118 435 1181 Standard deviation 8.51 30.75 54.36 9.2 36.6%3 72.04 CV (%) 6.6% 6.6% 4.5% 7.8% 8.1% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 129 466 1208 118 435 1181 Standard deviation 8.51 30.75 54.36 9.2 36.6%3 72.04 CV (%) 6.6% 6.6% 4.5% 7.8% 8.1% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 129 466 1208 118 435 1181 Mean (pg/ml) 129 466 1208 118 435 1181 Standard deviation 8.51 30.75 54.36 9.2 36.6%3 72.04 Standard deviation 8.51 30.75 54.36 9.2 36.6%3 72.04 CV (%) 6.6% 6.6% 4.5% 7.8% 8.1% 6.1% CV (%) 6.6% 6.6% 4.5% 7.8% 8.1% 6.1%

Sensitivity

<1 pg/mL

Function

Chemotactic activity for lymphocytes but not for monocytes or neutrophils.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

12517 MW

Specificity

Natural and recombinant human XCL1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Lymphotactin

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine gamma family.

Protein Name

Lymphotactin

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.112 0.16 0.302 0.514 0.977 1.686 2.343 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.112 0.16 0.302 0.514 0.977 1.686 2.343 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.112 0.16 0.302 0.514 0.977 1.686 2.343 O.D. 0.036 0.112 0.16 0.302 0.514 0.977 1.686 2.343

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.