Mouse IL-17F/Interleukin-17F ELISA Kit PicoKine®

Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 81 78 86 82 3.2 3.9% Sample 2 242 243 233 258 244 8.97 3.6% Sample 3 889 903 929 916 909 14.87 1.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 81 78 86 82 3.2 3.9% Sample 1 85 81 78 86 82 3.2 3.9% Sample 2 242 243 233 258 244 8.97 3.6% Sample 2 242 243 233 258 244 8.97 3.6% Sample 3 889 903 929 916 909 14.87 1.6% Sample 3 889 903 929 916 909 14.87 1.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 242 889 83 241 864 Standard deviation 5.01 14.76 68.45 4.98 17.7% 71.71 CV (%) 7.7%% 6.1% 7.7% 6% 6.6% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 242 889 83 241 864 Standard deviation 5.01 14.76 68.45 4.98 17.7% 71.71 CV (%) 7.7%% 6.1% 7.7% 6% 6.6% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 85 242 889 83 241 864 Mean (pg/ml) 85 242 889 83 241 864 Standard deviation 5.01 14.76 68.45 4.98 17.7% 71.71 Standard deviation 5.01 14.76 68.45 4.98 17.7% 71.71 CV (%) 7.7%% 6.1% 7.7% 6% 6.6% 8.3% CV (%) 7.7%% 6.1% 7.7% 6% 6.6% 8.3%

Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.052 0.082 0.148 0.3 0.605 1.281 2.421 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.052 0.082 0.148 0.3 0.605 1.281 2.421 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.052 0.082 0.148 0.3 0.605 1.281 2.421 O.D. 0.028 0.052 0.082 0.148 0.3 0.605 1.281 2.421

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.