Mouse IL-1RA/IL1RN ELISA Kit PicoKine®

Mouse IL-1RA/IL1RN ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il1rn in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization. IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 89 98 95 95 3.89 4% Sample 2 586 555 523 545 552 22.66 4.1% Sample 3 1056 1070 1157 1068 1087 40.33 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 99 89 98 95 95 3.89 4% Sample 1 99 89 98 95 95 3.89 4% Sample 2 586 555 523 545 552 22.66 4.1% Sample 2 586 555 523 545 552 22.66 4.1% Sample 3 1056 1070 1157 1068 1087 40.33 3.7% Sample 3 1056 1070 1157 1068 1087 40.33 3.7%

Synonyms

Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; IL1 inhibitor; Il1rn; Il-1ra

Gene Name

IL1RN

Gene ID

16181

UniProt

P25085

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R27-Q178

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 586 1056 92 570 1109 Standard deviation 4.95 36.91 47.52 6.25 42.18 64.32 CV (%) 5% 7.4% 4.5% 6.8% 7.4% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 99 586 1056 92 570 1109 Standard deviation 4.95 36.91 47.52 6.25 42.18 64.32 CV (%) 5% 7.4% 4.5% 6.8% 7.4% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 99 586 1056 92 570 1109 Mean (pg/ml) 99 586 1056 92 570 1109 Standard deviation 4.95 36.91 47.52 6.25 42.18 64.32 Standard deviation 4.95 36.91 47.52 6.25 42.18 64.32 CV (%) 5% 7.4% 4.5% 6.8% 7.4% 5.8% CV (%) 5% 7.4% 4.5% 6.8% 7.4% 5.8%

Sensitivity

<10 pg/mL

Function

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20274 MW

Specificity

Natural and recombinant mouse IL-1RA

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-1 receptor antagonist protein

Subcellular Location

Isoform 1: Secreted.

Protein Name

Interleukin-1 receptor antagonist protein

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.065 0.127 0.234 0.435 0.895 1.407 1.819 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.065 0.127 0.234 0.435 0.895 1.407 1.819 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.065 0.127 0.234 0.435 0.895 1.407 1.819 O.D. 0.002 0.065 0.127 0.234 0.435 0.895 1.407 1.819

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.