Human IL-1RA/IL1RN ELISA Kit PicoKine®

The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 52 59 58 56 2.69 4.8% Sample 2 296 274 294 294 289 8.98 3.1% Sample 3 803 845 834 932 853 47.86 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 52 59 58 56 2.69 4.8% Sample 1 57 52 59 58 56 2.69 4.8% Sample 2 296 274 294 294 289 8.98 3.1% Sample 2 296 274 294 294 289 8.98 3.1% Sample 3 803 845 834 932 853 47.86 5.6% Sample 3 803 845 834 932 853 47.86 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 296 803 56 318 731 Standard deviation 3.59 20.42 42.55 4.2 27.34 48.97 CV (%) 8.6% 6.9% 5.3% 7.5% 8.6% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 296 803 56 318 731 Standard deviation 3.59 20.42 42.55 4.2 27.34 48.97 CV (%) 8.6% 6.9% 5.3% 7.5% 8.6% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 296 803 56 318 731 Mean (pg/ml) 57 296 803 56 318 731 Standard deviation 3.59 20.42 42.55 4.2 27.34 48.97 Standard deviation 3.59 20.42 42.55 4.2 27.34 48.97 CV (%) 8.6% 6.9% 5.3% 7.5% 8.6% 6.7% CV (%) 8.6% 6.9% 5.3% 7.5% 8.6% 6.7%

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.105 0.177 0.294 0.534 0.941 1.501 2.226 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.105 0.177 0.294 0.534 0.941 1.501 2.226 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.105 0.177 0.294 0.534 0.941 1.501 2.226 O.D. 0.028 0.105 0.177 0.294 0.534 0.941 1.501 2.226

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.