Mouse IL-7/Interleukin-7 ELISA Kit PicoKine®

Mouse IL-7/Interleukin-7 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il7 in cell culture supernatants, serum and plasma (EDTA) . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 7 (IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7 (IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17 (T (H) 17) cells in experimental autoimmune encephalomyelitis (EAE) .2

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 59 35 54 50 2.47 5% Sample 2 173 242 246 236 224 7.31 3.3% Sample 3 551 757 917 821 762 34.8 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 59 35 54 50 2.47 5% Sample 1 51 59 35 54 50 2.47 5% Sample 2 173 242 246 236 224 7.31 3.3% Sample 2 173 242 246 236 224 7.31 3.3% Sample 3 551 757 917 821 762 34.8 4.6% Sample 3 551 757 917 821 762 34.8 4.6%

Synonyms

Interleukin-7; IL-7; Il7; Il-7

Gene Name

IL7

Gene ID

16196

UniProt

P10168

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E26-I154

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 173 551 56 201 599 Standard deviation 2.33 4.67 39.07 3.32 12.96 38.04 CV (%) 4.6 2.7% 7.1% 5.9% 6.5% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 173 551 56 201 599 Standard deviation 2.33 4.67 39.07 3.32 12.96 38.04 CV (%) 4.6 2.7% 7.1% 5.9% 6.5% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 51 173 551 56 201 599 Mean (pg/ml) 51 173 551 56 201 599 Standard deviation 2.33 4.67 39.07 3.32 12.96 38.04 Standard deviation 2.33 4.67 39.07 3.32 12.96 38.04 CV (%) 4.6 2.7% 7.1% 5.9% 6.5% 6.4% CV (%) 4.6 2.7% 7.1% 5.9% 6.5% 6.4%

Sensitivity

<1 pg/mL

Function

Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

17727 MW

Specificity

Natural and recombinant mouse IL-7

Anticoagulant

EDTA

Gene Name Synonym

Interleukin-7

Subcellular Location

Secreted.

Protein Name

Interleukin-7

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.144 0.227 0.368 0.579 1.005 1.578 2.446 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.144 0.227 0.368 0.579 1.005 1.578 2.446 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.144 0.227 0.368 0.579 1.005 1.578 2.446 O.D. 0.025 0.144 0.227 0.368 0.579 1.005 1.578 2.446

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.