Human IL-7/Interleukin-7 ELISA Kit PicoKine®

IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4. Interleukin 7 (IL7) is a protein5 that in humans is encoded by the IL7 gene.6, 7, 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V (D) J rearrangement of the T cell receptor beta (TCRB) during early T cell development.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 18 18 18 17 0.43 2.5% Sample 2 81 89 83 75 82 5 6% Sample 3 209 226 204 221 215 8.86 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 18 18 18 17 0.43 2.5% Sample 1 17 18 18 18 17 0.43 2.5% Sample 2 81 89 83 75 82 5 6% Sample 2 81 89 83 75 82 5 6% Sample 3 209 226 204 221 215 8.86 4.1% Sample 3 209 226 204 221 215 8.86 4.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 81 209 15 78 192 Standard deviation 0.98 5.50 9.82 1.00 5.38 10.56 CV (%) 5.8% 6.9% 4.7% 6.7% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 81 209 15 78 192 Standard deviation 0.98 5.50 9.82 1.00 5.38 10.56 CV (%) 5.8% 6.9% 4.7% 6.7% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 17 81 209 15 78 192 Mean (pg/ml) 17 81 209 15 78 192 Standard deviation 0.98 5.50 9.82 1.00 5.38 10.56 Standard deviation 0.98 5.50 9.82 1.00 5.38 10.56 CV (%) 5.8% 6.9% 4.7% 6.7% 6.9% 5.5% CV (%) 5.8% 6.9% 4.7% 6.7% 6.9% 5.5%

Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.034 0.092 0.152 0.272 0.491 0.949 1.594 2.331 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.034 0.092 0.152 0.272 0.491 0.949 1.594 2.331 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.034 0.092 0.152 0.272 0.491 0.949 1.594 2.331 O.D. 0.034 0.092 0.152 0.272 0.491 0.949 1.594 2.331

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.