Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine®

Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL6R in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL6R alpha (IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 48 54 48 51 3 5.8% Sample 2 271 254 262 234 255 13.66 5.3% Sample 3 821 928 965 877 897 54.21 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 48 54 48 51 3 5.8% Sample 1 54 48 54 48 51 3 5.8% Sample 2 271 254 262 234 255 13.66 5.3% Sample 2 271 254 262 234 255 13.66 5.3% Sample 3 821 928 965 877 897 54.21 6% Sample 3 821 928 965 877 897 54.21 6%

Synonyms

Interleukin 6 receptor ; Interleukin 6 receptor, isoform CRA_a ; IL6R ; hCG_1810911

Gene Name

IL6R

Gene ID

3570

UniProt

A0N0L5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-D358

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Interleukins, Lymphoid, Myeloid, Stem Cells, T Lymphocytic Lineage

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 271 821 59 259 773 Standard deviation 2.86 17.07 41.87 3.77 17.35 44.06 CV (%) 5.3% 6.7% 5.7% 6.4% 6.7% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 271 821 59 259 773 Standard deviation 2.86 17.07 41.87 3.77 17.35 44.06 CV (%) 5.3% 6.7% 5.7% 6.4% 6.7% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 54 271 821 59 259 773 Mean (pg/ml) 54 271 821 59 259 773 Standard deviation 2.86 17.07 41.87 3.77 17.35 44.06 Standard deviation 2.86 17.07 41.87 3.77 17.35 44.06 CV (%) 5.3% 6.7% 5.7% 6.4% 6.7% 5.7% CV (%) 5.3% 6.7% 5.7% 6.4% 6.7% 5.7%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

51548 MW

Specificity

Natural and recombinant human IL-6R alpha

Anticoagulant

Heparin, EDTA or Citrate

Gene Name Synonym

Interleukin 6 receptor

Protein Name

Interleukin-6 receptor subunit alpha

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.127 0.21 0.356 0.613 1.012 1.615 2.39 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.127 0.21 0.356 0.613 1.012 1.615 2.39 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.031 0.127 0.21 0.356 0.613 1.012 1.615 2.39 O.D. 0.031 0.127 0.21 0.356 0.613 1.012 1.615 2.39

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.