Human HBEGF ELISA Kit PicoKine®

HBEGF (Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 14 15 15 15 0.7 4.6% Sample 2 77 73 73 83 76 4.09 5.3% Sample 3 282 268 286 279 278 6.68 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 16 14 15 15 15 0.7 4.6% Sample 1 16 14 15 15 15 0.7 4.6% Sample 2 77 73 73 83 76 4.09 5.3% Sample 2 77 73 73 83 76 4.09 5.3% Sample 3 282 268 286 279 278 6.68 2.4% Sample 3 282 268 286 279 278 6.68 2.4%

Proheparin-binding EGF-like growth factor; Heparin-binding EGF-like growth factor; HB-EGF; HBEGF; Diphtheria toxin receptor; DT-R; HBEGF; DTR, DTS, HEGFL

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 77 282 15 70 280 Standard deviation 1 3.77 11.56 1.05 4.2 11.76 CV (%) 6% 4.9% 4.1% 7% 6% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 16 77 282 15 70 280 Standard deviation 1 3.77 11.56 1.05 4.2 11.76 CV (%) 6% 4.9% 4.1% 7% 6% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 16 77 282 15 70 280 Mean (pg/ml) 16 77 282 15 70 280 Standard deviation 1 3.77 11.56 1.05 4.2 11.76 Standard deviation 1 3.77 11.56 1.05 4.2 11.76 CV (%) 6% 4.9% 4.1% 7% 6% 4.2% CV (%) 6% 4.9% 4.1% 7% 6% 4.2%

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Heparin-binding EGF-like growth factor: Secreted, extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.036 0.081 0.12 0.2 0.369 0.6256 1.058 1.892 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.036 0.081 0.12 0.2 0.369 0.6256 1.058 1.892 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.036 0.081 0.12 0.2 0.369 0.6256 1.058 1.892 O.D. 0.036 0.081 0.12 0.2 0.369 0.6256 1.058 1.892

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.