Mouse TNFRSF18/GITR ELISA Kit PicoKine®

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+) CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 170 166 150 159 9.11 5.7% Sample 2 688 659 675 679 675 10.49 1.5% Sample 3 1008 1094 1052 1095 1062 35.8 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 150 170 166 150 159 9.11 5.7% Sample 1 150 170 166 150 159 9.11 5.7% Sample 2 688 659 675 679 675 10.49 1.5% Sample 2 688 659 675 679 675 10.49 1.5% Sample 3 1008 1094 1052 1095 1062 35.8 3.3% Sample 3 1008 1094 1052 1095 1062 35.8 3.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 688 1008 141 745 924 Standard deviation 9.75 44.03 47.37 9.58 53.64 50.82 CV (%) 6.5% 6.4% 4.7% 6.8% 7.2% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 688 1008 141 745 924 Standard deviation 9.75 44.03 47.37 9.58 53.64 50.82 CV (%) 6.5% 6.4% 4.7% 6.8% 7.2% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 150 688 1008 141 745 924 Mean (pg/ml) 150 688 1008 141 745 924 Standard deviation 9.75 44.03 47.37 9.58 53.64 50.82 Standard deviation 9.75 44.03 47.37 9.58 53.64 50.82 CV (%) 6.5% 6.4% 4.7% 6.8% 7.2% 5.5% CV (%) 6.5% 6.4% 4.7% 6.8% 7.2% 5.5%

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.114 0.227 0.336 0.556 0.913 1.473 1.998 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.114 0.227 0.336 0.556 0.913 1.473 1.998 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.114 0.227 0.336 0.556 0.913 1.473 1.998 O.D. 0.036 0.114 0.227 0.336 0.556 0.913 1.473 1.998

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.