Human TNFRSF18/GITR ELISA Kit PicoKine®

Human TNFRSF18/GITR ELISA Kit PicoKine® (96 Tests) . Quantitate Human TNFRSF18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+) CD4 (+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 135 136 127 144 135 6.02 4.4% Sample 2 705 808 735 671 729 50.53 6.9% Sample 3 1313 1243 1354 1475 1346 84.26 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 135 136 127 144 135 6.02 4.4% Sample 1 135 136 127 144 135 6.02 4.4% Sample 2 705 808 735 671 729 50.53 6.9% Sample 2 705 808 735 671 729 50.53 6.9% Sample 3 1313 1243 1354 1475 1346 84.26 6.2% Sample 3 1313 1243 1354 1475 1346 84.26 6.2%

Synonyms

Tumor necrosis factor receptor superfamily member 18; Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; CD357; TNFRSF18; AITR, GITR; UNQ319/PRO364

Gene Name

TNFRSF18

Gene ID

8784

UniProt

Q9Y5U5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q26-E161

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.

Applications

ELISA

Field of Research

Adaptive Immunity, Cancer, Cytokines, Growth Factors, Growth Factors/Hormones, Immunology, Innate Immunity, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 135 705 1313 138 657 1352 Standard deviation 7.15 40.89 101.1 8.83 42.7 108.16 CV (%) 5.3% 5.8% 7.7% 6.4% 6.5% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 135 705 1313 138 657 1352 Standard deviation 7.15 40.89 101.1 8.83 42.7 108.16 CV (%) 5.3% 5.8% 7.7% 6.4% 6.5% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 135 705 1313 138 657 1352 Mean (pg/ml) 135 705 1313 138 657 1352 Standard deviation 7.15 40.89 101.1 8.83 42.7 108.16 Standard deviation 7.15 40.89 101.1 8.83 42.7 108.16 CV (%) 5.3% 5.8% 7.7% 6.4% 6.5% 8% CV (%) 5.3% 5.8% 7.7% 6.4% 6.5% 8%

Sensitivity

<10 pg/mL

Function

Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

26000 MW

Specificity

Natural and recombinant human TNFRSF18

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 18

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Protein Name

Tumor necrosis factor receptor superfamily member 18

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.054 0.102 0.189 0.36 0.66 1.242 1.907 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.054 0.102 0.189 0.36 0.66 1.242 1.907 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.054 0.102 0.189 0.36 0.66 1.242 1.907 O.D. 0.002 0.054 0.102 0.189 0.36 0.66 1.242 1.907

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.