Mouse Galectin-3/LGALS3 ELISA Kit PicoKine®

Mouse Galectin-3/LGALS3 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Lgals3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 77 88 80 81 4.03 4.9% Sample 2 434 488 515 469 476 29.48 6.1% Sample 3 1619 1792 1773 1612 1699 83.8 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 81 77 88 80 81 4.03 4.9% Sample 1 81 77 88 80 81 4.03 4.9% Sample 2 434 488 515 469 476 29.48 6.1% Sample 2 434 488 515 469 476 29.48 6.1% Sample 3 1619 1792 1773 1612 1699 83.8 4.9% Sample 3 1619 1792 1773 1612 1699 83.8 4.9%

Synonyms

Galectin ; Lgals3 ; mCG_6908

Gene Name

LGALS3

Gene ID

16854

UniProt

Q8C253

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-I264

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cell Cycle, Cell Division, Oncoproteins, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 434 1619 80 402 1701 Standard deviation 8.1%1 31.24 126.28 7.68 32.56 153.09 CV (%) 7.8% 7.2% 7.8% 9.6% 8.1% 9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 81 434 1619 80 402 1701 Standard deviation 8.1%1 31.24 126.28 7.68 32.56 153.09 CV (%) 7.8% 7.2% 7.8% 9.6% 8.1% 9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 81 434 1619 80 402 1701 Mean (pg/ml) 81 434 1619 80 402 1701 Standard deviation 8.1%1 31.24 126.28 7.68 32.56 153.09 Standard deviation 8.1%1 31.24 126.28 7.68 32.56 153.09 CV (%) 7.8% 7.2% 7.8% 9.6% 8.1% 9% CV (%) 7.8% 7.2% 7.8% 9.6% 8.1% 9%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27415 MW

Specificity

Natural and recombinant mouse Galectin-3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Galectin

Protein Name

Galectin-3

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55 O.D. 0.033 0.129 0.207 0.406 0.715 1.21 2.012 2.55

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.