Human Galectin-3/LGALS3 ELISA Kit PicoKine®

Human Galectin-3/LGALS3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human LGALS3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 212 196 200 216 206 8.24 4% Sample 2 1876 2001 1983 1897 1939 53.64 2.7% Sample 3 4230 4475 3962 4238 4226 181.56 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 212 196 200 216 206 8.24 4% Sample 1 212 196 200 216 206 8.24 4% Sample 2 1876 2001 1983 1897 1939 53.64 2.7% Sample 2 1876 2001 1983 1897 1939 53.64 2.7% Sample 3 4230 4475 3962 4238 4226 181.56 4.2% Sample 3 4230 4475 3962 4238 4226 181.56 4.2%

Synonyms

Galectin-3; Gal-3;35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; LGALS3; MAC2

Gene Name

LGALS3

Gene ID

3958

UniProt

P17931

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-I250

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. .

Applications

ELISA

Field of Research

Cancer, Cell Cycle, Cell Division, Oncoproteins, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 212 1876 4230 205 1951 4307 Standard deviation 16.74 76.91 334.17 19.88 118.70 374.7 CV (%) 7.9% 4.1% 7.9% 9.7% 5.9% 8.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 212 1876 4230 205 1951 4307 Standard deviation 16.74 76.91 334.17 19.88 118.70 374.7 CV (%) 7.9% 4.1% 7.9% 9.7% 5.9% 8.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 212 1876 4230 205 1951 4307 Mean (pg/ml) 212 1876 4230 205 1951 4307 Standard deviation 16.74 76.91 334.17 19.88 118.70 374.7 Standard deviation 16.74 76.91 334.17 19.88 118.70 374.7 CV (%) 7.9% 4.1% 7.9% 9.7% 5.9% 8.7% CV (%) 7.9% 4.1% 7.9% 9.7% 5.9% 8.7%

Sensitivity

<10 pg/mL

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity) . In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

26152 MW

Specificity

Natural and recombinant human Galectin-3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Galectin-3

Subcellular Location

Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface.

Sequence Similarities

Contains 1 galectin domain.

Protein Name

Galectin-3

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.107 0.206 0.358 0.701 1.281 1.83 2.092 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.107 0.206 0.358 0.701 1.281 1.83 2.092 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.107 0.206 0.358 0.701 1.281 1.83 2.092 O.D. 0.001 0.107 0.206 0.358 0.701 1.281 1.83 2.092

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.