Mouse Galectin-1 ELISA Kit PicoKine®

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 316 342 346 336 12.07 3.5% Sample 2 1747 1764 1871 1625 1751 87.26 4.9% Sample 3 4774 4662 4642 4948 4756 121.46 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 316 342 346 336 12.07 3.5% Sample 1 343 316 342 346 336 12.07 3.5% Sample 2 1747 1764 1871 1625 1751 87.26 4.9% Sample 2 1747 1764 1871 1625 1751 87.26 4.9% Sample 3 4774 4662 4642 4948 4756 121.46 2.5% Sample 3 4774 4662 4642 4948 4756 121.46 2.5%

Galectin-1; Gal-1;14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; S-Lac lectin 1; Lgals1; Gbp

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 1747 4774 318 1697 4684 Standard deviation 22.29 132.77 300.76 20.67 144.24 304.46 CV (%) 6.5% 7.6% 8.5% 6.5% 8.5% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 1747 4774 318 1697 4684 Standard deviation 22.29 132.77 300.76 20.67 144.24 304.46 CV (%) 6.5% 7.6% 8.5% 6.5% 8.5% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 343 1747 4774 318 1697 4684 Mean (pg/ml) 343 1747 4774 318 1697 4684 Standard deviation 22.29 132.77 300.76 20.67 144.24 304.46 Standard deviation 22.29 132.77 300.76 20.67 144.24 304.46 CV (%) 6.5% 7.6% 8.5% 6.5% 8.5% 6.5% CV (%) 6.5% 7.6% 8.5% 6.5% 8.5% 6.5%

May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity. Strong inducer of T-cell apoptosis (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457 O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.