Human ErbB-2 ELISA Kit PicoKine®

HER2/neu (also known as ErbB-2) stands for Human Epidermal growth factor Receptor 2 and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340 (cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family.1 The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17 (17q21-q22) 2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 164 178 168 153 165 8.95 5.4% Sample 2 544 528 493 539 526 19.91 3.7% Sample 3 1478 1506 1587 1640 1552 64.34 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 164 178 168 153 165 8.95 5.4% Sample 1 164 178 168 153 165 8.95 5.4% Sample 2 544 528 493 539 526 19.91 3.7% Sample 2 544 528 493 539 526 19.91 3.7% Sample 3 1478 1506 1587 1640 1552 64.34 4.1% Sample 3 1478 1506 1587 1640 1552 64.34 4.1%

Receptor tyrosine-protein kinase erbB-2;2.7.10.1; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; ERBB2; HER2, MLN19, NEU, NGL

Cancer, Cell Type Markers, Growth Factors, Growth Factors/Hormones, Oncoproteins, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tags & Cell Markers, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 164 544 1478 167 559 1477 Standard deviation 12.79 38.62 93.11 16.03 40.8 104.86 CV (%) 7.8% 7.1% 7.3%% 9.6% 7.3% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 164 544 1478 167 559 1477 Standard deviation 12.79 38.62 93.11 16.03 40.8 104.86 CV (%) 7.8% 7.1% 7.3%% 9.6% 7.3% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 164 544 1478 167 559 1477 Mean (pg/ml) 164 544 1478 167 559 1477 Standard deviation 12.79 38.62 93.11 16.03 40.8 104.86 Standard deviation 12.79 38.62 93.11 16.03 40.8 104.86 CV (%) 7.8% 7.1% 7.3%% 9.6% 7.3% 7.1% CV (%) 7.8% 7.1% 7.3%% 9.6% 7.3% 7.1%

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs) . Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.07 0.108 0.184 0.427 0.66 1.378 2.095 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.07 0.108 0.184 0.427 0.66 1.378 2.095 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.07 0.108 0.184 0.427 0.66 1.378 2.095 O.D. 0.034 0.07 0.108 0.184 0.427 0.66 1.378 2.095

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.