Mouse ESM1/Endocan ELISA Kit PicoKine®

Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 53 49 52 52 2.5 4.8% Sample 2 169 162 178 184 173 8.4 4.8% Sample 3 683 675 706 643 676 22.56 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 56 53 49 52 52 2.5 4.8% Sample 1 56 53 49 52 52 2.5 4.8% Sample 2 169 162 178 184 173 8.4 4.8% Sample 2 169 162 178 184 173 8.4 4.8% Sample 3 683 675 706 643 676 22.56 3.3% Sample 3 683 675 706 643 676 22.56 3.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 169 683 60 163 636 Standard deviation 3.36 10.14 52.59 4.2 12.22 56.6 CV (%) 6% 6% 7.7% 7% 7.5% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 56 169 683 60 163 636 Standard deviation 3.36 10.14 52.59 4.2 12.22 56.6 CV (%) 6% 6% 7.7% 7% 7.5% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 56 169 683 60 163 636 Mean (pg/ml) 56 169 683 60 163 636 Standard deviation 3.36 10.14 52.59 4.2 12.22 56.6 Standard deviation 3.36 10.14 52.59 4.2 12.22 56.6 CV (%) 6% 6% 7.7% 7% 7.5% 8.9% CV (%) 6% 6% 7.7% 7% 7.5% 8.9%

Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.071 0.124 0.179 0.283 0.498 0.88 1.609 2.399 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.071 0.124 0.179 0.283 0.498 0.88 1.609 2.399 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 Concentration (pg/ml) 0 23.4 46.8 93.7 187.5 375 750 1500 O.D. 0.071 0.124 0.179 0.283 0.498 0.88 1.609 2.399 O.D. 0.071 0.124 0.179 0.283 0.498 0.88 1.609 2.399

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.