Human ESM1/Endocan ELISA Kit PicoKine®

Human ESM1/Endocan ELISA Kit PicoKine® (96 Tests) . Quantitate Human ESM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 83 83 80 84 3.67 4.3% Sample 2 269 235 269 222 248 20.76 8.3% Sample 3 832 815 926 825 849 44.57 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 83 83 80 84 3.67 4.3% Sample 1 90 83 83 80 84 3.67 4.3% Sample 2 269 235 269 222 248 20.76 8.3% Sample 2 269 235 269 222 248 20.76 8.3% Sample 3 832 815 926 825 849 44.57 5.2% Sample 3 832 815 926 825 849 44.57 5.2%

Synonyms

Endothelial cell-specific molecule 1; ESM-1; ESM1

Gene Name

ESM1

Gene ID

11082

UniProt

Q9NQ30

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: W20-R184

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.

Applications

ELISA

Field of Research

Cell Type Markers, Tags & Cell Markers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 269 832 96 293 860 Standard deviation 7.11 14.79 45.76 8.44 19.33 54.18 CV (%) 7.9% 5.5% 5.5% 8.8% 6.6% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 269 832 96 293 860 Standard deviation 7.11 14.79 45.76 8.44 19.33 54.18 CV (%) 7.9% 5.5% 5.5% 8.8% 6.6% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 269 832 96 293 860 Mean (pg/ml) 90 269 832 96 293 860 Standard deviation 7.11 14.79 45.76 8.44 19.33 54.18 Standard deviation 7.11 14.79 45.76 8.44 19.33 54.18 CV (%) 7.9% 5.5% 5.5% 8.8% 6.6% 6.3% CV (%) 7.9% 5.5% 5.5% 8.8% 6.6% 6.3%

Sensitivity

<10 pg/mL

Function

Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

20095 MW

Specificity

Natural and recombinant human ESM1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Endothelial cell-specific molecule 1

Subcellular Location

Secreted.

Protein Name

Endothelial cell-specific molecule 1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.079 0.117 0.196 0.335 0.596 1.061 1.836 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.079 0.117 0.196 0.335 0.596 1.061 1.836 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.079 0.117 0.196 0.335 0.596 1.061 1.836 O.D. 0.027 0.079 0.117 0.196 0.335 0.596 1.061 1.836

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.