Human EMMPRIN/CD147/BSG ELISA Kit PicoKine®

Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147 (cluster of differentiation 147) . It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition (Miyauchi et al., 1991; Kanekura et al., 1991) .This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 69 64 65 66 2.27 3.4% Sample 2 232 198 221 210 215 12.63 5.8% Sample 3 1065 992 955 1022 1008 40.33 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 69 64 65 66 2.27 3.4% Sample 1 69 69 64 65 66 2.27 3.4% Sample 2 232 198 221 210 215 12.63 5.8% Sample 2 232 198 221 210 215 12.63 5.8% Sample 3 1065 992 955 1022 1008 40.33 4% Sample 3 1065 992 955 1022 1008 40.33 4%

Basigin;5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; EMMPRIN; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF; CD147; BSG; UNQ6505/PRO21383

Present only in vascular endothelium in non- neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Neuroscience, Pathways and Processes, Sensory System, Signal Transduction, Visual System

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 232 1065 70 210 975 Standard deviation 7.64 16.24 76.68 5.32 15.54 77.02 CV (%) 7.6% 7% 7.2% 7.6% 7.4% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 232 1065 70 210 975 Standard deviation 7.64 16.24 76.68 5.32 15.54 77.02 CV (%) 7.6% 7% 7.2% 7.6% 7.4% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 69 232 1065 70 210 975 Mean (pg/ml) 69 232 1065 70 210 975 Standard deviation 7.64 16.24 76.68 5.32 15.54 77.02 Standard deviation 7.64 16.24 76.68 5.32 15.54 77.02 CV (%) 7.6% 7% 7.2% 7.6% 7.4% 7.9% CV (%) 7.6% 7% 7.2% 7.6% 7.4% 7.9%

Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS) . Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane ; Single-pass type I membrane protein . Melanosome . Colocalizes with SLC16A1 and SLC16A8. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.102 0.152 0.257 0.429 0.735 1.234 2.018 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.102 0.152 0.257 0.429 0.735 1.234 2.018 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.102 0.152 0.257 0.429 0.735 1.234 2.018 O.D. 0.028 0.102 0.152 0.257 0.429 0.735 1.234 2.018

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.