Human Decorin / DCN ELISA Kit PicoKine®

Human Decorin / DCN ELISA Kit PicoKine® (96 Tests) . Quantitate Human DCN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS) .Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 51 50 59 55 4.52 8.2% Sample 2 247 279 302 287 278 20.1 7.2% Sample 3 1001 962 922 976 965 28.61 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 51 50 59 55 4.52 8.2% Sample 1 60 51 50 59 55 4.52 8.2% Sample 2 247 279 302 287 278 20.1 7.2% Sample 2 247 279 302 287 278 20.1 7.2% Sample 3 1001 962 922 976 965 28.61 2.9% Sample 3 1001 962 922 976 965 28.61 2.9%

Synonyms

Decorin; Bone proteoglycan II; PG-S2; PG40; DCN; SLRR1B

Gene Name

DCN

Gene ID

1634

UniProt

P07585

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G17-K359

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Monoclonal antibody from mouse

Applications

ELISA

Field of Research

Collagen, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Growth Factors/Hormones, Signal Transduction, Structures

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 247 1001 56 270 1067 Standard deviation 4.56 16.54 70.07 4.31 20.25 88.56 CV (%) 7.6% 6.7% 7% 7.7% 7.5% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 247 1001 56 270 1067 Standard deviation 4.56 16.54 70.07 4.31 20.25 88.56 CV (%) 7.6% 6.7% 7% 7.7% 7.5% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 247 1001 56 270 1067 Mean (pg/ml) 60 247 1001 56 270 1067 Standard deviation 4.56 16.54 70.07 4.31 20.25 88.56 Standard deviation 4.56 16.54 70.07 4.31 20.25 88.56 CV (%) 7.6% 6.7% 7% 7.7% 7.5% 8.3% CV (%) 7.6% 6.7% 7% 7.7% 7.5% 8.3%

Sensitivity

<10 pg/mL

Function

May affect the rate of fibrils formation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

39747 MW

Specificity

Natural and recombinant human Decorin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Decorin

Subcellular Location

Secreted, extracellular space, extracellular matrix.

Sequence Similarities

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Protein Name

Decorin

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.183 0.281 0.45 0.71 1.024 1.607 2.411 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.183 0.281 0.45 0.71 1.024 1.607 2.411 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.183 0.281 0.45 0.71 1.024 1.607 2.411 O.D. 0.007 0.183 0.281 0.45 0.71 1.024 1.607 2.411

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.