Mouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine®

C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 34 32 29 31 1.8 5.8% Sample 2 182 204 199 194 194 8.16 4.2% Sample 3 491 507 522 441 490 30.47 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 34 32 29 31 1.8 5.8% Sample 1 31 34 32 29 31 1.8 5.8% Sample 2 182 204 199 194 194 8.16 4.2% Sample 2 182 204 199 194 194 8.16 4.2% Sample 3 491 507 522 441 490 30.47 6.2% Sample 3 491 507 522 441 490 30.47 6.2%

Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles) .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5%

Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in ing the migration of B-lymphocytes to follicles in secondary lymphoid organs.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.031 0.102 0.178 0.329 0.708 1.163 1.851 2.431 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.031 0.102 0.178 0.329 0.708 1.163 1.851 2.431 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.031 0.102 0.178 0.329 0.708 1.163 1.851 2.431 O.D. 0.031 0.102 0.178 0.329 0.708 1.163 1.851 2.431

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.