Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine®

Human CXCL13 / BLC / BCA1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CXCL13 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 31 36 31 32 2.06 6.4% Sample 2 216 244 229 223 228 10.31 4.5% Sample 3 441 424 416 416 424 10.2 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 31 36 31 32 2.06 6.4% Sample 1 32 31 36 31 32 2.06 6.4% Sample 2 216 244 229 223 228 10.31 4.5% Sample 2 216 244 229 223 228 10.31 4.5% Sample 3 441 424 416 416 424 10.2 2.4% Sample 3 441 424 416 416 424 10.2 2.4%

Synonyms

C-X-C motif chemokine 13; Angie; B cell-attracting chemokine 1; BCA-1; B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; CXCL13; BCA1, BLC, SCYB13

Gene Name

CXCL13

Gene ID

10563

UniProt

O43927

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V23-R94

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 216 441 30 232 416 Standard deviation 2.2 16.84 24.69 2.19 20.64 27.3%8 CV (%) 6.9% 7.8% 5.6% 7.3% 8.9% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 216 441 30 232 416 Standard deviation 2.2 16.84 24.69 2.19 20.64 27.3%8 CV (%) 6.9% 7.8% 5.6% 7.3% 8.9% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 216 441 30 232 416 Mean (pg/ml) 32 216 441 30 232 416 Standard deviation 2.2 16.84 24.69 2.19 20.64 27.3%8 Standard deviation 2.2 16.84 24.69 2.19 20.64 27.3%8 CV (%) 6.9% 7.8% 5.6% 7.3% 8.9% 6.8% CV (%) 6.9% 7.8% 5.6% 7.3% 8.9% 6.8%

Sensitivity

<1 pg/mL

Function

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B- lymphocytes. Binds to BLR1/CXCR5.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

12664 MW

Specificity

Natural and recombinant human CXCL13

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-X-C motif chemokine 13

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Protein Name

C-X-C motif chemokine 13

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.097 0.166 0.292 0.491 0.934 1.51 2.126 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.097 0.166 0.292 0.491 0.934 1.51 2.126 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.097 0.166 0.292 0.491 0.934 1.51 2.126 O.D. 0.025 0.097 0.166 0.292 0.491 0.934 1.51 2.126

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.