Human CXCL10/IP-10 ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (gamma-IP10 or IP-10) . CXCL10 is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.It is a potent inhibitor of angiogenesis in vivo. The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines. It is a RAS target gene and is overexpressed in the majority of colorectal cancers.This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 54 60 64 59 3.57 6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 3 879 923 848 978 907 48.89 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 54 60 64 59 3.57 6% Sample 1 60 54 60 64 59 3.57 6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 3 879 923 848 978 907 48.89 5.3% Sample 3 879 923 848 978 907 48.89 5.3%

C-X-C motif chemokine 10;10 kDa interferon gamma-induced protein; Gamma-IP10; IP-10; Small-inducible cytokine B10; CXCL10 (1-73) ; CXCL10; INP10, SCYB10

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.136 0.206 0.345 0.535 0.998 1.625 2.684 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.136 0.206 0.345 0.535 0.998 1.625 2.684 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.038 0.136 0.206 0.345 0.535 0.998 1.625 2.684 O.D. 0.038 0.136 0.206 0.345 0.535 0.998 1.625 2.684

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.