Human CXCL9/Mig ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG) . CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4.1, 2 CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3.3 The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 94 97 93 92 4.84 5.2% Sample 2 277 262 265 250 263 9.6 3.6% Sample 3 773 838 880 794 821 41.23 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 94 97 93 92 4.84 5.2% Sample 1 84 94 97 93 92 4.84 5.2% Sample 2 277 262 265 250 263 9.6 3.6% Sample 2 277 262 265 250 263 9.6 3.6% Sample 3 773 838 880 794 821 41.23 5% Sample 3 773 838 880 794 821 41.23 5%

C-X-C motif chemokine 9; Gamma-interferon-induced monokine; Monokine induced by interferon-gamma; HuMIG; MIG; Small-inducible cytokine B9; CXCL9; CMK, MIG, SCYB9

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 277 773 77 272 823 Standard deviation 6.55 11.63 57.2 6.16 11.69 60.9 CV (%) 7.8% 4.2% 7.4% 8% 4.3% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 277 773 77 272 823 Standard deviation 6.55 11.63 57.2 6.16 11.69 60.9 CV (%) 7.8% 4.2% 7.4% 8% 4.3% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 84 277 773 77 272 823 Mean (pg/ml) 84 277 773 77 272 823 Standard deviation 6.55 11.63 57.2 6.16 11.69 60.9 Standard deviation 6.55 11.63 57.2 6.16 11.69 60.9 CV (%) 7.8% 4.2% 7.4% 8% 4.3% 7.4% CV (%) 7.8% 4.2% 7.4% 8% 4.3% 7.4%

Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.095 0.161 0.26 0.437 0.781 1.401 2.374 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.095 0.161 0.26 0.437 0.781 1.401 2.374 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.095 0.161 0.26 0.437 0.781 1.401 2.374 O.D. 0.02 0.095 0.161 0.26 0.437 0.781 1.401 2.374

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.