Human CXCL5/LIX/ENA-78 ELISA Kit PicoKine®

C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78) . It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 77 65 68 69 4.74 6.8% Sample 2 270 257 287 288 275 12.85 4.6% Sample 3 964 912 900 813 897 54.26 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 77 65 68 69 4.74 6.8% Sample 1 66 77 65 68 69 4.74 6.8% Sample 2 270 257 287 288 275 12.85 4.6% Sample 2 270 257 287 288 275 12.85 4.6% Sample 3 964 912 900 813 897 54.26 6% Sample 3 964 912 900 813 897 54.26 6%

C-X-C motif chemokine 5; ENA-78 (1-78) ; Epithelial-derived neutrophil-activating protein 78; Neutrophil-activating peptide ENA-78; Small-inducible cytokine B5; ENA-78 (8-78) ; ENA-78 (9-78) ; CXCL5; ENA78, SCYB5

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 270 964 61 293 970 Standard deviation 2.77 18.09 74.22 3.53 22.26 82.45 CV (%) 4.2% 6.7% 7.7% 5.8% 7.6% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 270 964 61 293 970 Standard deviation 2.77 18.09 74.22 3.53 22.26 82.45 CV (%) 4.2% 6.7% 7.7% 5.8% 7.6% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 66 270 964 61 293 970 Mean (pg/ml) 66 270 964 61 293 970 Standard deviation 2.77 18.09 74.22 3.53 22.26 82.45 Standard deviation 2.77 18.09 74.22 3.53 22.26 82.45 CV (%) 4.2% 6.7% 7.7% 5.8% 7.6% 8.5% CV (%) 4.2% 6.7% 7.7% 5.8% 7.6% 8.5%

Involved in neutrophil activation. In vitro, ENA-78 (8- 78) and ENA-78 (9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.091 0.147 0.246 0.486 0.827 1.557 2.91 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.091 0.147 0.246 0.486 0.827 1.557 2.91 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.091 0.147 0.246 0.486 0.827 1.557 2.91 O.D. 0.024 0.091 0.147 0.246 0.486 0.827 1.557 2.91

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.