Rat CXCL1/Gro Alpha ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha) . In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 34 32 34 1.92 5.6% Sample 2 226 219 236 214 223 8.25 3.6% Sample 3 428 378 438 405 412 23.11 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 34 32 34 1.92 5.6% Sample 1 36 37 34 32 34 1.92 5.6% Sample 2 226 219 236 214 223 8.25 3.6% Sample 2 226 219 236 214 223 8.25 3.6% Sample 3 428 378 438 405 412 23.11 5.6% Sample 3 428 378 438 405 412 23.11 5.6%

Growth-regulated alpha protein; C-X-C motif chemokine 1; Cytokine-induced neutrophil chemoattractant 1; CINC-1; Platelet-derived growth factor-inducible protein KC; Cxcl1; Cinc1, Gro, Scyb1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 226 428 36 217 466 Standard deviation 1.58 9.26 26.1 1.58 9.33 33.55 CV (%) 4.4% 4.1% 6.1% 4.4% 4.3% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 226 428 36 217 466 Standard deviation 1.58 9.26 26.1 1.58 9.33 33.55 CV (%) 4.4% 4.1% 6.1% 4.4% 4.3% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 226 428 36 217 466 Mean (pg/ml) 36 226 428 36 217 466 Standard deviation 1.58 9.26 26.1 1.58 9.33 33.55 Standard deviation 1.58 9.26 26.1 1.58 9.33 33.55 CV (%) 4.4% 4.1% 6.1% 4.4% 4.3% 7.2% CV (%) 4.4% 4.1% 6.1% 4.4% 4.3% 7.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.079 0.174 0.258 0.407 0.684 1.056 1.57 2.074 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.079 0.174 0.258 0.407 0.684 1.056 1.57 2.074 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.079 0.174 0.258 0.407 0.684 1.056 1.57 2.074 O.D. 0.079 0.174 0.258 0.407 0.684 1.056 1.57 2.074

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.