Mouse CXCL1/Gro Alpha ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-alpha) . In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 31 27 28 1.47 5.2% Sample 2 242 253 228 272 248 16.08 6.4% Sample 3 501 480 502 525 502 15.92 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 31 27 28 1.47 5.2% Sample 1 28 29 31 27 28 1.47 5.2% Sample 2 242 253 228 272 248 16.08 6.4% Sample 2 242 253 228 272 248 16.08 6.4% Sample 3 501 480 502 525 502 15.92 3.1% Sample 3 501 480 502 525 502 15.92 3.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 242 501 27 253 536 Standard deviation 1.42 16.45 37.07 1.67 21.25 45.02 CV (%) 8.4%% 6.8% 7.4% 6.2% 8.4% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 242 501 27 253 536 Standard deviation 1.42 16.45 37.07 1.67 21.25 45.02 CV (%) 8.4%% 6.8% 7.4% 6.2% 8.4% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 242 501 27 253 536 Mean (pg/ml) 28 242 501 27 253 536 Standard deviation 1.42 16.45 37.07 1.67 21.25 45.02 Standard deviation 1.42 16.45 37.07 1.67 21.25 45.02 CV (%) 8.4%% 6.8% 7.4% 6.2% 8.4% 8.4% CV (%) 8.4%% 6.8% 7.4% 6.2% 8.4% 8.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.024 0.068 0.106 0.189 0.35 0.613 1.207 2.037 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.024 0.068 0.106 0.189 0.35 0.613 1.207 2.037 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.024 0.068 0.106 0.189 0.35 0.613 1.207 2.037 O.D. 0.024 0.068 0.106 0.189 0.35 0.613 1.207 2.037

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.