Mouse CTLA4 ELISA Kit PicoKine®

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152 (Cluster of differentiation 152), is a protein receptor that down regulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 45 53 59 51 1.43 2.8% Sample 2 235 241 203 226 226 13.35 5.9% Sample 3 1015 1163 1075 1234 1122 50.48 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 45 53 59 51 1.43 2.8% Sample 1 47 45 53 59 51 1.43 2.8% Sample 2 235 241 203 226 226 13.35 5.9% Sample 2 235 241 203 226 226 13.35 5.9% Sample 3 1015 1163 1075 1234 1122 50.48 4.5% Sample 3 1015 1163 1075 1234 1122 50.48 4.5%

Adaptive Immunity, Autoimmune, Hematopoietic Progenitors, Immune System Diseases, Immunology, Lymphoid, Non-CD, Stem Cells, T Cells, T Lymphocytic Lineage

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 235 1015 51 244 1069 Standard deviation 2.63 10.11 69.58 3.11 11.71 70.55 CV (%) 5.6% 4.3% 6.5% 6.1% 4.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 235 1015 51 244 1069 Standard deviation 2.63 10.11 69.58 3.11 11.71 70.55 CV (%) 5.6% 4.3% 6.5% 6.1% 4.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 47 235 1015 51 244 1069 Mean (pg/ml) 47 235 1015 51 244 1069 Standard deviation 2.63 10.11 69.58 3.11 11.71 70.55 Standard deviation 2.63 10.11 69.58 3.11 11.71 70.55 CV (%) 5.6% 4.3% 6.5% 6.1% 4.8% 6.6% CV (%) 5.6% 4.3% 6.5% 6.1% 4.8% 6.6%

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.066 0.111 0.21 0.381 0.713 1.296 2.267 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.066 0.111 0.21 0.381 0.713 1.296 2.267 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.066 0.111 0.21 0.381 0.713 1.296 2.267 O.D. 0.011 0.066 0.111 0.21 0.381 0.713 1.296 2.267

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.