Human IL7R/CD127 ELISA Kit PicoKine®

Human IL7R/CD127 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL7R in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7) . The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D) J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 410 395 383 426 403 16.13 4% Sample 2 2617 2389 2451 2237 2423 136.17 5.6% Sample 3 9061 9810 8729 10576 9544 712.96 7.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 410 395 383 426 403 16.13 4% Sample 1 410 395 383 426 403 16.13 4% Sample 2 2617 2389 2451 2237 2423 136.17 5.6% Sample 2 2617 2389 2451 2237 2423 136.17 5.6% Sample 3 9061 9810 8729 10576 9544 712.96 7.4% Sample 3 9061 9810 8729 10576 9544 712.96 7.4%

Synonyms

Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; CD127; IL7R

Gene Name

IL7R

Gene ID

3575

UniProt

P16871

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-I262

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Interleukins, Lymphoid, Myeloid, Stem Cells, T Lymphocytic Lineage

Standard

20 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 410 2617 9061 443 2807 9233 Standard deviation 25.42 120.38 516.47 33.22 140.35 627.84 CV (%) 6.2% 4.6% 5.7% 7.5% 5% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 410 2617 9061 443 2807 9233 Standard deviation 25.42 120.38 516.47 33.22 140.35 627.84 CV (%) 6.2% 4.6% 5.7% 7.5% 5% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 410 2617 9061 443 2807 9233 Mean (pg/ml) 410 2617 9061 443 2807 9233 Standard deviation 25.42 120.38 516.47 33.22 140.35 627.84 Standard deviation 25.42 120.38 516.47 33.22 140.35 627.84 CV (%) 6.2% 4.6% 5.7% 7.5% 5% 6.8% CV (%) 6.2% 4.6% 5.7% 7.5% 5% 6.8%

Sensitivity

<10 pg/mL

Function

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

51581 MW

Specificity

Natural and recombinant human IL7R

Gene Name Synonym

Interleukin-7 receptor subunit alpha

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the type I cytokine receptor family. Type 4 subfamily.

Protein Name

Interleukin-7 receptor subunit alpha

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.059 0.098 0.167 0.274 0.59 1.061 1.929 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.059 0.098 0.167 0.274 0.59 1.061 1.929 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.032 0.059 0.098 0.167 0.274 0.59 1.061 1.929 O.D. 0.032 0.059 0.098 0.167 0.274 0.59 1.061 1.929

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.