Human CD40/TNFRSF5 ELISA Kit PicoKine®

CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 34 33 2.23 6.7% Sample 2 167 185 189 177 179 8.41 4.6% Sample 3 443 505 507 519 493 29.64 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 34 33 2.23 6.7% Sample 1 32 30 36 34 33 2.23 6.7% Sample 2 167 185 189 177 179 8.41 4.6% Sample 2 167 185 189 177 179 8.41 4.6% Sample 3 443 505 507 519 493 29.64 6% Sample 3 443 505 507 519 493 29.64 6%

Adaptive Immunity, Atherosclerosis, B Cells, Cardiovascular, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 167 443 32 177 404 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 167 443 32 177 404 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 167 443 32 177 404 Mean (pg/ml) 32 167 443 32 177 404 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6% CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6%

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.