Human CD40/TNFRSF5 ELISA Kit PicoKine®

Human CD40/TNFRSF5 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD40 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. . Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 34 33 2.23 6.7% Sample 2 167 185 189 177 179 8.41 4.6% Sample 3 443 505 507 519 493 29.64 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 30 36 34 33 2.23 6.7% Sample 1 32 30 36 34 33 2.23 6.7% Sample 2 167 185 189 177 179 8.41 4.6% Sample 2 167 185 189 177 179 8.41 4.6% Sample 3 443 505 507 519 493 29.64 6% Sample 3 443 505 507 519 493 29.64 6%

Synonyms

Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD40; CD40; TNFRSF5

Gene Name

CD40

Gene ID

958

UniProt

P25942

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-R193

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

B-cells and in primary carcinomas.

Applications

ELISA

Field of Research

Adaptive Immunity, Atherosclerosis, B Cells, Cardiovascular, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 167 443 32 177 404 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 167 443 32 177 404 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 167 443 32 177 404 Mean (pg/ml) 32 167 443 32 177 404 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 Standard deviation 1.66 9.18 34.99 1.92 10.79 38.78 CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6% CV (%) 5.2% 5.5% 7.9% 6% 6.1% 9.6%

Sensitivity

<1 pg/mL

Function

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30619 MW

Specificity

Natural and recombinant human CD40

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 5

Subcellular Location

Isoform I: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 4 TNFR-Cys repeats.

Protein Name

Tumor necrosis factor receptor superfamily member 5

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952 O.D. 0.032 0.078 0.149 0.245 0.413 0.751 1.246 1.952

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.