Human CD36/SR-B3/GP4 ELISA Kit PicoKine®

CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb) . The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 329 358 360 320 341 17.55 5.1% Sample 2 1274 1304 1194 1283 1263 41.71 3.3% Sample 3 5223 5923 6080 5774 5750 322.92 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 329 358 360 320 341 17.55 5.1% Sample 1 329 358 360 320 341 17.55 5.1% Sample 2 1274 1304 1194 1283 1263 41.71 3.3% Sample 2 1274 1304 1194 1283 1263 41.71 3.3% Sample 3 5223 5923 6080 5774 5750 322.92 5.6% Sample 3 5223 5923 6080 5774 5750 322.92 5.6%

Platelet glycoprotein 4; Fatty acid translocase; FAT; Glycoprotein IIIb; GPIIIB; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; Platelet glycoprotein IV; GPIV; Thrombospondin receptor; CD36; CD36; GP3B, GP4

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 329 1274 5223 298 1195 5251 Standard deviation 23.03 76.44 360.38 21.45 81.26 456.83 CV (%) 7% 6% 6.9% 7.2% 6.8% 8.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 329 1274 5223 298 1195 5251 Standard deviation 23.03 76.44 360.38 21.45 81.26 456.83 CV (%) 7% 6% 6.9% 7.2% 6.8% 8.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 329 1274 5223 298 1195 5251 Mean (pg/ml) 329 1274 5223 298 1195 5251 Standard deviation 23.03 76.44 360.38 21.45 81.26 456.83 Standard deviation 23.03 76.44 360.38 21.45 81.26 456.83 CV (%) 7% 6% 6.9% 7.2% 6.8% 8.7% CV (%) 7% 6% 6.9% 7.2% 6.8% 8.7%

Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL) . May function as a cell adhesion molecule. ly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. As a coreceptor for TLR4-TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.053 0.1 0.171 0.324 0.592 1.076 1.817 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.053 0.1 0.171 0.324 0.592 1.076 1.817 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.053 0.1 0.171 0.324 0.592 1.076 1.817 O.D. 0.029 0.053 0.1 0.171 0.324 0.592 1.076 1.817

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.