Mouse TNFRSF7/CD27 ELISA Kit PicoKine®

CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 88 88 94 88 3.89 4.4% Sample 2 588 678 590 656 628 39.77 6.3% Sample 3 1891 1745 2088 2100 1956 147.39 7.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 88 88 94 88 3.89 4.4% Sample 1 83 88 88 94 88 3.89 4.4% Sample 2 588 678 590 656 628 39.77 6.3% Sample 2 588 678 590 656 628 39.77 6.3% Sample 3 1891 1745 2088 2100 1956 147.39 7.5% Sample 3 1891 1745 2088 2100 1956 147.39 7.5%

Adaptive Immunity, Cancer, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Cells, T Lymphocytic Lineage, TNF Superfamily, Tumor Immunology

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 588 1891 74 556 2053 Standard deviation 3.81 45.86 115.35 3.55 51.15 149.86 CV (%) 4.6% 7.8% 6.1% 4.8% 9.2% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 588 1891 74 556 2053 Standard deviation 3.81 45.86 115.35 3.55 51.15 149.86 CV (%) 4.6% 7.8% 6.1% 4.8% 9.2% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 83 588 1891 74 556 2053 Mean (pg/ml) 83 588 1891 74 556 2053 Standard deviation 3.81 45.86 115.35 3.55 51.15 149.86 Standard deviation 3.81 45.86 115.35 3.55 51.15 149.86 CV (%) 4.6% 7.8% 6.1% 4.8% 9.2% 7.3% CV (%) 4.6% 7.8% 6.1% 4.8% 9.2% 7.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.012 0.107 0.187 0.402 0.747 1.268 1.599 2.006 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.012 0.107 0.187 0.402 0.747 1.268 1.599 2.006 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.012 0.107 0.187 0.402 0.747 1.268 1.599 2.006 O.D. 0.012 0.107 0.187 0.402 0.747 1.268 1.599 2.006

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.