Mouse CD14 ELISA Kit PicoKine®

Mouse CD14 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Cd14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1809 1708 1610 1761 1722 73.87 4.2% Sample 2 4760 4159 4699 4655 4568 239.2 5.2% Sample 3 24340 25258 25930 26104 25408 692.82 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1809 1708 1610 1761 1722 73.87 4.2% Sample 1 1809 1708 1610 1761 1722 73.87 4.2% Sample 2 4760 4159 4699 4655 4568 239.2 5.2% Sample 2 4760 4159 4699 4655 4568 239.2 5.2% Sample 3 24340 25258 25930 26104 25408 692.82 2.7% Sample 3 24340 25258 25930 26104 25408 692.82 2.7%

Synonyms

Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14; Cd14

Gene Name

CD14

Gene ID

12475

UniProt

P10810

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-P345

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Type Markers, Hematopoietic Progenitors, Immunology, Innate Immunity, Mesenchymal Stem Cells, Myeloid, Stem Cells, TLR Signaling

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1809 4760 24340 1781 4305 24426 Standard deviation 103.11 261.8 1217 119.32 236.77 1392.28 CV (%) 5.7% 5.5% 5% 6.7% 5.5% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1809 4760 24340 1781 4305 24426 Standard deviation 103.11 261.8 1217 119.32 236.77 1392.28 CV (%) 5.7% 5.5% 5% 6.7% 5.5% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1809 4760 24340 1781 4305 24426 Mean (pg/ml) 1809 4760 24340 1781 4305 24426 Standard deviation 103.11 261.8 1217 119.32 236.77 1392.28 Standard deviation 103.11 261.8 1217 119.32 236.77 1392.28 CV (%) 5.7% 5.5% 5% 6.7% 5.5% 5.7% CV (%) 5.7% 5.5% 5% 6.7% 5.5% 5.7%

Sensitivity

<10 pg/mL

Function

In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

39204 MW

Specificity

Natural and recombinant mouse CD14

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Monocyte differentiation antigen CD14

Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Name

Monocyte differentiation antigen CD14

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.052 0.15 0.278 0.42 0.649 1.111 1.605 1.941 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.052 0.15 0.278 0.42 0.649 1.111 1.605 1.941 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.052 0.15 0.278 0.42 0.649 1.111 1.605 1.941 O.D. 0.052 0.15 0.278 0.42 0.649 1.111 1.605 1.941

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.