Human CCL27/CTACK ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK) . This gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 98 95 92 92 4.43 4.8% Sample 2 240 249 256 245 247 5.85 2.3% Sample 3 846 852 955 890 885 43.39 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 98 95 92 92 4.43 4.8% Sample 1 86 98 95 92 92 4.43 4.8% Sample 2 240 249 256 245 247 5.85 2.3% Sample 2 240 249 256 245 247 5.85 2.3% Sample 3 846 852 955 890 885 43.39 4.9% Sample 3 846 852 955 890 885 43.39 4.9%

C-C motif chemokine 27; CC chemokine ILC; Cutaneous T-cell-attracting chemokine; CTACK; ESkine; IL-11 R-alpha-locus chemokine; Skinkine; Small-inducible cytokine A27; CCL27; ILC, SCYA27

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 240 846 85 239 777 Standard deviation 4.98 10.56 60.06 5.78 13.86 62.93 CV (%) 5.8% 4.4% 7.1% 6.8% 5.8% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 240 846 85 239 777 Standard deviation 4.98 10.56 60.06 5.78 13.86 62.93 CV (%) 5.8% 4.4% 7.1% 6.8% 5.8% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 86 240 846 85 239 777 Mean (pg/ml) 86 240 846 85 239 777 Standard deviation 4.98 10.56 60.06 5.78 13.86 62.93 Standard deviation 4.98 10.56 60.06 5.78 13.86 62.93 CV (%) 5.8% 4.4% 7.1% 6.8% 5.8% 8.1% CV (%) 5.8% 4.4% 7.1% 6.8% 5.8% 8.1%

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.135 0.191 0.292 0.467 0.78 1.249 2.057 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.135 0.191 0.292 0.467 0.78 1.249 2.057 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.135 0.191 0.292 0.467 0.78 1.249 2.057 O.D. 0.067 0.135 0.191 0.292 0.467 0.78 1.249 2.057

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.