Human CCL18/PARC ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine) . CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 17 18 18 17 0.43 2.5% Sample 2 49 56 49 48 50 3.2 6.4% Sample 3 269 295 278 296 284 11.45 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 17 18 18 17 0.43 2.5% Sample 1 18 17 18 18 17 0.43 2.5% Sample 2 49 56 49 48 50 3.2 6.4% Sample 2 49 56 49 48 50 3.2 6.4% Sample 3 269 295 278 296 284 11.45 4% Sample 3 269 295 278 296 284 11.45 4%

C-C motif chemokine 18; Alternative macrophage activation-associated CC chemokine 1; AMAC-1; CC chemokine PARC; Dendritic cell chemokine 1; DC-CK1; Macrophage inflammatory protein 4; MIP-4; Pulmonary and activation-regulated chemokine; Small-inducible cytokine A18; CCL18 (1-68) ; CCL18 (3-69) ; CCL18 (4-69) ; CCL18; AMAC1, DCCK1, MIP4, PARC, SCYA18

Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 49 269 18 52 279 Standard deviation 0.99 3.52 20.17 1.29 4.62 22.59 CV (%) 5.5% 7.2% 7.5% 7.2% 8.9% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 49 269 18 52 279 Standard deviation 0.99 3.52 20.17 1.29 4.62 22.59 CV (%) 5.5% 7.2% 7.5% 7.2% 8.9% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 49 269 18 52 279 Mean (pg/ml) 18 49 269 18 52 279 Standard deviation 0.99 3.52 20.17 1.29 4.62 22.59 Standard deviation 0.99 3.52 20.17 1.29 4.62 22.59 CV (%) 5.5% 7.2% 7.5% 7.2% 8.9% 8.1% CV (%) 5.5% 7.2% 7.5% 7.2% 8.9% 8.1%

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.008 0.103 0.175 0.316 0.624 0.992 1.6 2.067 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.008 0.103 0.175 0.316 0.624 0.992 1.6 2.067 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.008 0.103 0.175 0.316 0.624 0.992 1.6 2.067 O.D. 0.008 0.103 0.175 0.316 0.624 0.992 1.6 2.067

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.