Mouse CCL17/TARC ELISA Kit PicoKine®

Mouse CCL17/TARC ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ccl17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC) . CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 56 56 60 57 1.78 3.1% Sample 2 209 190 231 207 209 14.56 6.9% Sample 3 996 963 968 951 969 16.5 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 56 56 60 57 1.78 3.1% Sample 1 59 56 56 60 57 1.78 3.1% Sample 2 209 190 231 207 209 14.56 6.9% Sample 2 209 190 231 207 209 14.56 6.9% Sample 3 996 963 968 951 969 16.5 1.7% Sample 3 996 963 968 951 969 16.5 1.7%

Synonyms

C-C motif chemokine ; Ccl17

Gene Name

CCL17

Gene ID

20295

UniProt

F6R5P4

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-P93

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 209 996 56 226 1076 Standard deviation 3.18 13.58 57.76 3.64 17.17 75.32 CV (%) 5.4% 6.5% 5.8% 6.5% 7.6% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 209 996 56 226 1076 Standard deviation 3.18 13.58 57.76 3.64 17.17 75.32 CV (%) 5.4% 6.5% 5.8% 6.5% 7.6% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 209 996 56 226 1076 Mean (pg/ml) 59 209 996 56 226 1076 Standard deviation 3.18 13.58 57.76 3.64 17.17 75.32 Standard deviation 3.18 13.58 57.76 3.64 17.17 75.32 CV (%) 5.4% 6.5% 5.8% 6.5% 7.6% 7% CV (%) 5.4% 6.5% 5.8% 6.5% 7.6% 7%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

11539 MW

Specificity

Natural and recombinant mouse CCL17

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine

Subcellular Location

Secreted .

Sequence Similarities

Belongs to the intercrine beta (chemokine CC) family.

Protein Name

C-C motif chemokine 17

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.117 0.178 0.305 0.533 0.875 1.457 2.061 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.117 0.178 0.305 0.533 0.875 1.457 2.061 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.117 0.178 0.305 0.533 0.875 1.457 2.061 O.D. 0.054 0.117 0.178 0.305 0.533 0.875 1.457 2.061

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.